abstract：:We describe a gas chromatographic method for measuring submicrogram quantities of dianhydrogalactitol, a promising antineoplastic agent (currently undergoing clinical trials in humans), in plasma. The drug is first extracted from blood plasma by saturating the aqueous phase with solid potassium carbonate and extractin...

journal_title：Clinical chemistry

authors： Kimura T,Sternson LA,Higuchi T

更新日期：1976-10-01 00:00:00

abstract：:Ireport a detailed series of microcalorimetric measurement of glucose concentrations in five reference samples of serum. The method utilized (a) calibration in the actual medium of analysis and (b) a correction for interferences owing to the nonspecificity of the enzyme hexokinase. The microcalorimetric results are co...

journal_title：Clinical chemistry

authors： Goldberg RN

更新日期：1976-10-01 00:00:00

abstract：:Clinicians experience difficulty in correctly interpreting the results of in vitro thyroid function tests in the presence of abnormalities of thyrobinding proteins or when results are borderline. This difficulty has been largely resolved in our laboratory by three innovations. First, the borderline areas for each of t...

journal_title：Clinical chemistry

authors： Pain RW,Duncan BM

更新日期：1976-10-01 00:00:00

abstract：:Ion chromatography is a new analytical technique in which the ions to be analyzed are separated on a special pellicular ion-exchange column. Once separated, the effluent from the separating column passes into a second ion-exchange column, which removes the eluent or buffer ions from the moving phase without removing t...

journal_title：Clinical chemistry

authors： Anderson C

更新日期：1976-09-01 00:00:00

abstract：:We describe a simple method in which a water-soluble mixture of triocatanoin and a surfactant, "Triton X-114," are used in preparing solutions of triglycerides (triacylglycerols) in either human serum, solutions of albumin, or water. Analytical recovery added triglyceride was quantitative by two methods. The addition ...

journal_title：Clinical chemistry

authors： Bonderman DP,Proksch GJ,Bonderman PW

更新日期：1976-08-01 00:00:00

abstract：:We describe an automated determination of serum hexosamine by the Elson-Morgan reaction, together with reagent modifications that minimiz interference from amino acids and sugars present in acid hydrolysates of sera. We used a novel 15-min autoclave procedure for the acid hydrolysis of sera before analysis to facilita...

journal_title：Clinical chemistry

authors： Sobocinski PZ,Canterbury WJ,Jurgens KH

更新日期：1976-08-01 00:00:00

abstract：:We determined estrogen receptor protein and carcinoembryonic antigen in cytosols prepared from 189 human breast carcinoma tissues, 85 benign or normal breast biopsies, and 101 tissue specimens metastatic from breast carcinoma. Carcinoembryonic antigen was observed in 70% of the primary carcinomatous tissues, 15% of th...

journal_title：Clinical chemistry

authors： Menendez-Botet CJ,Nisselbaum JS,Fleisher M,Rosen PP,Fracchia A,Robbins G,Urban JA,Schwartz MK

更新日期：1976-08-01 00:00:00

abstract：:We describe a process for preparing a cholesterol-rich lipoprotein extract from bovine serum. The material is used to enhance cholestrol concentration up to 3.5 g/liter in a clear control material from human serum. The optical clarity of the enriched serum is only slightly affected by lyophilization and reconstitution...

journal_title：Clinical chemistry

authors： Proksch GJ,Bonderman DP

更新日期：1976-08-01 00:00:00

abstract：:Determination of the concentration of erythrocyte folate by means of competitive protein binding assay critically depends on the extraction procedure applied. Results will be influenced by variable factors such as the in vitro age of the blood samples, the degree of hemolysis, the presence of ascorbic acid, and the pH...

journal_title：Clinical chemistry

authors： Mortensen E

更新日期：1976-07-01 00:00:00

abstract：:Lyophilized serum specimens were manufactured in such a way that fixed quantitative interspecimen relationships were established. The specimens were analyzed for about twenty constituents by several thousand laboratories in 1971, 1972, and 1973. Most of the consensus mean values derived from these analyses were found ...

journal_title：Clinical chemistry

authors： Grannis GF

更新日期：1976-07-01 00:00:00

abstract：:We describe a sensitive and precise gas-chromatographic method, in which cytosine is used as the internal standard, for determination of an antifungal agent, 5-fluorocytosine, in serum. The trimethylsilyl derivative of this drug is well separated from the internal standard and from normal serum constituents. Amphoteri...

journal_title：Clinical chemistry

authors： Harding SA,Johnson GF,Solomon HM

更新日期：1976-06-01 00:00:00

abstract：:Toxicological determinations are crucial to coroners' or medical examiners' judgments that drugs are significantly involved in a death. However, differences in laboratory procedures, thoroughness of screening, and limits of detection may result in artifactual differences in the toxicological results and the subsequent...

journal_title：Clinical chemistry

authors： Dinovo EC,Gottschalk LA

更新日期：1976-06-01 00:00:00

abstract：:Serum specimens from patients on primidone therapy were assayed by two currently available procedures: a commercially available enzyme immunoassay (EMIT) and a published gas-chromatographic procedure. Both procedures were done with commercially available materials and equipment. Results by the two procedures agreed we...

journal_title：Clinical chemistry

authors： Sun L,Walwick ER

更新日期：1976-06-01 00:00:00

abstract：:A profile (chromatographic pattern) of the neutral and acidic metabolites present in cerebrospinal fluid from newborns and infants was obtained by gas-liquid chromatography. The metabolites are those extracted with ethyl acetate and diethyl ether. They are converted to trimethylsilyl ether derivatives, and chromatogra...

journal_title：Clinical chemistry

authors： Malcolm RD,Leonards R

更新日期：1976-05-01 00:00:00

abstract：:Both cellulose acetate electrophoresis and citrate agar electrophoresis were performed on 834 blood samples collected on filter paper in Jamaica and shipped for testing to the National Hemoglobinopathy Standardization Laboratory at the U.S. National Center for Disease Control. Additionally, 30 blood samples collected ...

journal_title：Clinical chemistry

authors： Schmidt RM,Brosious EM,Holland S,Wright JM,Serjeant GR

更新日期：1976-05-01 00:00:00

abstract：:To establish optimum conditions for creatine kinase (EC 2.7.3.2) activity measurement with the creatine phosphate in equilibrium creatine reaction, we re-examined all kinetics factors relevant to an optimal and standardized enzyme assay at 30 and 25 degrees C. We determined the pH optimum in vaious buffers, considerin...

journal_title：Clinical chemistry

authors： Szasz G,Gruber W,Bernt E

更新日期：1976-05-01 00:00:00

abstract：:I describe an assay for serum estriol during pregnancy, which does not require prior extraction or chromatography of the serum. Fifty microliters of 20-fold diluted serum can be assayed to detect as little as 20 pg of estriol. For a 20 mug/liter serum estriol concentration the CV was 16%; for 100 mug/liter concentrati...

journal_title：Clinical chemistry

authors： Standefer J

更新日期：1976-04-01 00:00:00

abstract：:The isoenzymes of lactate dehydrogenase (EC 1.1.1.27) were separated with excellent resolution in less than 1 h by high-pressure anion-exchange chromatography. These isoenzymes were determined in some tissue extracts and high-activity serum samples by this technique. ...

journal_title：Clinical chemistry

authors： Kudirka PJ,Schroeder RR,Hewitt TE,Toren EC Jr

更新日期：1976-04-01 00:00:00

abstract：:We describe a rapid, reliable radioimmunoassay for unconjugated estriol in plasma. Polyethylene glycol (Carbowax 6000) is used to separate antibody-bound and free steroid. The assay is sensitive (25 pg for standards), precise, and accurate. At high and low concentrations of estriol, intra-assay coefficients of variati...

journal_title：Clinical chemistry

authors： Schiller HS,Brammall MA

更新日期：1976-03-01 00:00:00

abstract：:We present a comparative evaluation of two commercial kits, the "Quantitope" and "GammaCoat," for radioimmunoassay of digoxin in serum. These kits, in which iodine-125 is used as a label, proved to suitable for digoxin assay as determined by their reproducibility, sensitivity, precision, and a regression analysis. Hem...

journal_title：Clinical chemistry

authors： Kuczala ZJ,Ahluwalia GS

更新日期：1976-02-01 00:00:00

abstract：:A direct mehtod [Clin. Chim. Acta 46, 113 (1973)] for determination of inorganic phosphate in serum was adapted for use with a centrifugal analyzer. Contamination is minimized and analysis rate maximized by doing the reaction in the reagent wells of the transfer disc and by utilizing the high-speed spectrophotometric ...

journal_title：Clinical chemistry

authors： Wentz PW,Savory J,Cross RE

更新日期：1976-02-01 00:00:00

abstract：:We describe a chromatographic system involving a high-performance chemically-bonded reverse-phase column and fluorescence detection for measurement of indoles in urine. We controlled retention and selectivity by optimizing the methanol content and pH of the mobile phase. Six reference indoles were separated in less th...

journal_title：Clinical chemistry

authors： Graffeo AP,Karger BL

更新日期：1976-02-01 00:00:00

abstract：:I describe a simple, single-tube batch fractionation procedure for separating MM and MB isoenzymes of creatine kinase on a macroporous strong anion exchanger (AG MP-1, Bio-Rad Laboratories). The isoenzymes can be separated in less than 3 min, with a resulting dilution of the serum with no more than an equal volume of ...

journal_title：Clinical chemistry

authors： Morin LG

更新日期：1976-01-01 00:00:00

abstract：:The pattern of organic acids in urine from 15 normal newborn infants was investigated by gas chromatography/mass spectrometry of trimethylsilylated derivatives. The urine contains large amounts of succinic, fumaric, 2-ketoglutaric, and 3-hydroxy-3-methylglutaric acids, which are all fairly small components of urine fr...

journal_title：Clinical chemistry

authors： Björkman L,McLean C,Steen G

更新日期：1976-01-01 00:00:00

abstract：:Six alpha-amylase (EC 3.2.1.1) isoenzymes have been resolved electrophoretically on cellulose acetate membranes in a discontinuous buffer system. The fastest migrating isoenzymes are of salivary origin (S1, S2, S3), the slower ones of pancreatic origin (P1, P2, P3). We determined the amylase isoenzyme distribution in ...

journal_title：Clinical chemistry

authors： Legaz ME,Kenny MA

更新日期：1976-01-01 00:00:00

abstract：:We describe a simple, rapid fluorometric assay for separate quantitative analysis of procainamide and N-acetylprocainamide in mixtures. The effective lenear range (fluorescence vs. concentration) in serum is 0.1 to 10.0 mg/liter, regardless of the ratio (by weight) of the two drugs from 1:10 to 10:1. Analytical recove...

journal_title：Clinical chemistry

authors： Matusik E,Gibson TP

更新日期：1975-12-01 00:00:00

abstract：:Agarose column chromatography has been used to separate plasma lipoproteins into very-low-density lipoproteins (VLDL), low-density lipoproteins (LDL) and high-density lipoproteins (HDL). Applied to the diagnosis of primary type III hyperlipoproteinemia, the procedure is capable of demonstrating three characteristic an...

journal_title：Clinical chemistry

authors： Shepherd J,Packard CJ,Dryburgh FJ,Third JL

更新日期：1975-12-01 00:00:00

abstract：:With use of a specially designed gradient-generating system, of anion-exchange resins of 10-20 mum particle diameter, and of an orcinol solution in concentrated sulfuric acid (1 g/liter) as detection reagent, a mixture of 16 carbohydrates can be separated in less than 4 h. The limit of detection for most sugars is in ...

journal_title：Clinical chemistry

authors： Voelter W,Bauer H

更新日期：1975-12-01 00:00:00

abstract：:Erythrocyte and plasma cholinesterase activities were measured biweekly in one group of 22 subjects for a year and daily for three weeks in another group of nine men. The average range [i.e., (range/mean) X 100] of activity of erythrocyte cholinesterase in men during a year was 8% and during three weeks was 5%. For pl...

journal_title：Clinical chemistry

authors： Sidell FR,Kaminskis A

更新日期：1975-12-01 00:00:00

abstract：:We report here the development of a new method that allows continuous determination of the oxygen dissociation curve for microsamples (600 mul) of whole blood under conditions of pH, pCO2, methemoglobin concentration, and 2,3-diphosphoglycerate content closely approaching those found in the circulatory system. The met...

journal_title：Clinical chemistry

authors： Rossi-Bernardi L,Luzzana M,Samaja M,Davi M,DaRiva-Ricci D,Minoli J,Seaton B,Berger RL

更新日期：1975-11-01 00:00:00

abstract：:We describe an enzymatic fluorometric method for determining glucose concentrations in blood samples by analysis on a semi-solid surface (silicone-rubber pads). The method is an adaptation of existing spectrophotometric procedures based on the sequential coupling reactions with glucose-6-phosphate dehydrogenase and NA...

journal_title：Clinical chemistry

authors： Kiang SW,Kuan JW,Guilbault GG

更新日期：1975-11-01 00:00:00

abstract：:The method is based on the selective activating capacity of dithiothreitol on creatine kinase isoenzyme MB, after isoenzyme MM is activated by glutathione. Isolated isoenzymes MM and MB of human and canine origin were assayed individually and in mixtures of known activities. When glutathione was present in the assay m...

journal_title：Clinical chemistry

authors： Rao PS,Lukes JJ,Ayres SM,Mueller H

更新日期：1975-10-01 00:00:00

abstract：:Kappa Bence Jones proteinuria was found by immunoelectrophoretic techniques in a patient with plasma cell leukemia, who presented with no M-proteins either in serum or urine. However, a significant decrease in normal immunoglobulins was observed. On microscopic examination of kidney sections obtained a autopsy, protei...

journal_title：Clinical chemistry

authors： Chow C,Spitzer RW

更新日期：1975-10-01 00:00:00

abstract：:As the domain of monitoring therapeutic drugs rapidly expands from experimental investigations to everyday patient care, all clinical laboratory scientists, but especially clinical chemists, must learn to deal with their involvement in this expansion. First, the theoretical concepts underlying clinical pharmacology mu...

journal_title：Clinical chemistry

authors： Werner M,Sutherland EW 3rd,Abramson FP

更新日期：1975-09-01 00:00:00

abstract：:We assessed several methods of inorganic phosphate assay for their suitability in estimating phospholipids in digested extracts of amniotic fluids. The extraction and digestion procedures used for phospholipids from amniotic fluid were also examined critically. The effect of contamination by blood or obstetric cream h...

journal_title：Clinical chemistry

authors： Badham LP,Worth HG

更新日期：1975-09-01 00:00:00

abstract：:Measurement of serum ascorbate may be useful in long-term population studies because of the possible influence of ascorbate on numerous physiological factors. We describe an automated method for determining ascorbate in serum and urine by using the reduction of ferric iron by ascorbate and the formation of a color bet...

journal_title：Clinical chemistry

authors： Butts WC,Mulvihill HJ

更新日期：1975-09-01 00:00:00

abstract：:We report the adaptation of four radioassays to the prototype of an automated radioimmunoassay system ("Centria," Union Carbide). The system consists of three integrated modules: (a) an automated pipettor, which dispenses samples and reagents; (b) the key module, an incubator/separator, in which centrifugal force is u...

journal_title：Clinical chemistry

authors： Ertingshausen G,Shapiro SI,Green G,Zborowski G

更新日期：1975-08-01 00:00:00

abstract：:A new computer-interfaced system of general applicability in the clinical laboratory has been built. The system is constructed around a new type multicuvette. Quantitation is currently by spectrophotometry and is on-line, with use of a mini-computer with output going to an electrostatic printer/plotter. The system can...

journal_title：Clinical chemistry

authors： Vestergaard P,Bachman A

更新日期：1975-08-01 00:00:00

abstract：:Because of uncertainty as to the molecular weight of transferrin, a previous comparison [Von der Heul et al., Clin. Chim. Acta 38, 347 (1972)] between transferrin content of serum and total iron-binding capacity cannot be definitive. We found a conversion factor for expressing the maximum amount of iron bound by 1 mg ...

journal_title：Clinical chemistry

authors： Tsung SH,Rosenthal WA,Milewski KA

更新日期：1975-07-01 00:00:00

abstract：:A reaction of urea, o-phthalaldehyde and N-(1-naphthyl)ethylenediamine is described for measurement of urea by manual, continuous-flow, and kinetic methods. The continuous-flow system requires 25 mu-l of sample; 40 samples can be analyzed per hour. The kinetic method requires no enzymes, has no lag phase, and has good...

journal_title：Clinical chemistry

authors： Jung D,Biggs H,Erikson J,Ledyard PU

更新日期：1975-07-01 00:00:00